›› 2011, Vol. 23 ›› Issue (4): 298-301.doi: 10.3969/j.issn.1004-616x.2011.04.013

• 论著 • Previous Articles     Next Articles

In vitro anti-tumor effect of BLT2 receptor antagonist LY255283

SUN Xiao-li1，HAN Chun-guang1，WANG Min2，WANG Li-jie，LI Hai-bing，GAO Zhi-qing，WANG Qiong，LIU Yong-xue*   

  1. 1. Institute of Radiation Medicine, Academy of Military Medical Sciences of PLA, Beijing 100850; 2. Out-patient Department, No. 92117 Forces of PLA, Beijing 100072, China
  • Received:2011-01-18 Revised:2011-05-02 Online:2011-07-30 Published:2011-07-30
  • Contact: LIU Yong-xue

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Abstract: OBJECTIVE: To study the inhibition effect of LY255283，a BLT2 receptor antagonist，on different human cancer cell lines. METHODS：The expressions of BLT2 receptor in SMMC7721、A549、Bel7402、BGC823、Eca109、HeLa、HL60、MCF7 and Pc3 cell were evaluated by RT-PCR. The inhibition effect of LY255283 on the nine tumor cell lines was analyzed by MTT assay. Apoptotic cells were evaluated by flow cytometry and fluorescence microscopy. RESULTS：All nine tumor cell lines expressed BLT2 receptors. LY255283 inhibited the proliferation of all nine tumor cell lines，the IC50 values were 0.21，11.17，1.28，5.9，7.9，1.59，0.14，1.25 and 31.28 μmol/L， respectively. The apoptotic rates of MCF-7 cells induced by LY255283 at 1 and 10 μmol/L were 20.47% and 31.28%，respectively，which were significantly increased compared with blank control group (8.66%) and DMSO control group (11.71%) (P<0.01). CONCLUSION：BLT2 receptor was expressed in nine human tumor cell lines，and the specific antagonist LY255283 could inhibit the proliferation of tumor cells probably by inducing apoptosis.

Key words: leukotriene B4, BLT2, LY255283, tumor cells